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The potential clinical utility of circulating tumor cells ctcs in the diagnosis and management of cancer has drawn a lot of attention in the past 10 years.Ctcs disseminate from tumors into the bloodstream and are believed to carry vital information about tumor onset, progression, and metastasis.In addition, ctcs reflect different biological aspects of the primary tumor they originate from.
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The wire is placed intravenously, allowing it to screen large blood volumes during the 30-min collection period.The wire successfully captured ctcs in 92 2224 of cancer with a median of 5.5 ctcs range 050 and 16 ctcs range 2515 detected in breast and non-small cell lung cancer patients, respectively.
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Hypothesizing that the limited blood volume commonly used for ctc analysis 110 ml accounts for variable detection rates, we used leukapheresis to screen large blood volumes for ctcs.This enabled a more reliable detection of ctcs at high frequency even in nonmetastatic cancer patients.
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In 2,183 blood samples from 964 metastatic carcinoma patients, ctcs ranged from 0 to 23,618 ctcs per 7.5 ml mean, 60 - 693 ctcs per 7.5 ml, and 36 781 of 2,183 of the specimens had 2 ctcs.
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The wire is placed intravenously, allowing it to screen large blood volumes during the 30min collection period.The wire successfully captured ctcs in 92 2224 of cancer with a median of 5.5 ctcs range 050 and 16 ctcs range 2515 detected in breast and.
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A similar proportion of ctcs and tumor cells expressed epcam, at 50 and 53, respectively.30 of ctcs and 14 of tumor cells maintained expression of epcam even upon loss of ecad fig.
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Magnetic cell separation has become a popular technique to enrich or deplete cells of interest from a heterogeneous cell population.One important aspect of magnetic cell separation is the degree to which a cell binds paramagnetic material.It is this paramagnetic material that imparts a positive magnetophoretic mobility to the target cell, thus allowing effective cell separation.
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Microfluidic devices have had a significant impact on the field of biomedical diagnostics, and are widely used throughout the drug development, drug delivery and bio-medical research industries.The diminutive scale of the flow channels in microfluidic systems increases the surface-to-volume ratio, and is thus advantageous for many biomedical applications ,.
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Rapid Separation Of Human Breast Cancer Cells From
Circulating tumor cells ctcs are nomadic cells shed into peripheral blood from a primary tumor.As ctcs can be analyzed for early cancer detection and treatment monitoring, they play an.
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Besides ctcs, however, the capture of other circulating cells, such as trophoblasts, has been demonstrated to provide prenatal diagnostics.181 capturing other biocomponents from the blood, such as exosomes 182 or circulating dna, 183 also provides additional personalized molecular information about a patient.However, not all target cells are.
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Blood, a complex biological fluid, comprises 45 cellular components suspended in protein rich plasma.These different hematologic components perform distinct functions in vivo and thus the ability to efficiently fractionate blood into its individual components has innumerable applications in both clinical diagnosis and biological research.Yet, processing blood is not trivial.
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